Distribution of triple fluorescent recombinant HSV-1 (rHSV35R22Y48C) proteins in a host cell


Vero cells were infected with rHSV35R22Y48C, and visualized at different times post infection by confocal laser scaning microscopy. The rHSV35R22Y48C virus encodes glycoprotein H fused to enhanced yellow fluorescent protein (gH-EYFP), tegument-VP16 Protein fused to enhanced cyan fluorescent protein (ECFP-VP16), and the capsid-VP26 protein fused to monomeric red fluorescent protein (mRFP-VP26).